Table of Contents
Table of Contents
This book is dedicated to the memory of Dr Berend Houwen and Dr Noriyuki Tatsumi
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Library of Congress Cataloging-in-Publication Data
Laboratory hematology practice / edited by Kandice Kottke-Marchant, Bruce H. Davis.
p. ; cm.
Includes bibliographical references and index.
ISBN-13: 978-1-4051-6218-0 (hard cover : alk. paper)
ISBN-10: 1-4051-6218-X (hard cover : alk. paper)
1. Hematology. 2. Blood–Analysis. I. Kottke-Marchant, Kandice. II. Davis, Bruce H., M.D.
[DNLM: 1. Hematologic Tests–methods. 2. Blood Chemical Analysis–methods. 3. Cytological Techniques. 4. Hematologic Diseases–diagnosis. QY 400]
RB45.L235 2012
616.07'561–dc23
2011023990
A catalogue record for this book is available from the British Library.
Wiley also publishes its books in a variety of electronic formats. Some content that appears in print may not be available in electronic books.
Contributors
Dorothy M. Adcock MD
Medical Director
Esoterix Inc.
Englewood, CO, USA
Robert W. Allan MD
Clinical Associate Professor
Department of Pathology and Laboratory Medicine
University of Florida College of Medicine
Gainesville, FL, USA
Julia Almeida MD, PhD
Associate Professor
Servicio General de Citometría and Departamento de Medicina
Instituto de Biología Celular y Molecular del Cáncer
Centro de Investigación del Cáncer/IBMCC (CSIC-USAL)
Universidad de Salamanca
Salamanca, Spain
Samer Z. Al-Quran MD
Associate Professor
Department of Pathology, Immunology and Laboratory Medicine
University of Florida College of Medicine
Gainesville, FL, USA
Ivan Alvarez-Twose MD
Clinical Associate Professor
Instituto de Estudios de Mastocitosis de Castilla La Mancha
Hospital Virgen del Valle
Toledo, Spain
Diane C. Arthur MD
Staff Clinician
Head, Clinical Cytogenetics Section
Laboratory of Pathology, CCR, NCI, NIH
Bethesda, MD, USA
C. Bruce Bagwell MD, PhD
President
Verity Software House
Topsham, ME, USA
Barbara J. Bain MBBS, FRACP, FRCPath
Professor of Diagnostic Haematology
Imperial College Faculty of Medicine
St Mary’s Hospital
London, UK
Patrick W. Barnes MA MT(ASCP)
Laboratory Manager—Hematology
Barnes-Jewish Hospital
St. Louis, MO, USA
Marie-Christine Béné PharmSciD, PhD
Immunology Professor and Biologist
Centre Hospitalier Universitaire et Faculté de Médecine de Nancy
Nancy Université
Nancy, France
David J. Blomberg MD
Formerly Associate Professor, Pathology and Laboratory Medicine, Univ. of MN, Duluth, School of Medicine.
Formerly of Arrowhead Pathologists, P.A.
Duluth, MN, USA
Bobby L. Boyanton Jr. MD
Associate Professor of Pathology
Oakland University William Beaumont School of Medicine
Medical Director, Clinical Microbiology
Associate Medical Director, Molecular Pathology
Beaumont Hospital
Department of Clinical Pathology
Royal Oak, MI, USA
Raul C. Braylan MD
Professor Emeritus
Department of Pathology and Laboratory Medicine
University of Florida College of Medicine
Gainesville, FL, USA
Carol Briggs BSc, FIBMS
Chief Biomedical Scientist and Researcher
Department of Haematology
University College London Hospitals
London, UK
Lesley J. Bruce PhD
Senior Research Scientist
Bristol Institute for Transfusion Sciences
NHS Blood & Transplant
Bristol, UK
Layna K. Cardel BS, MT(ASCP)
Education Specialist
Mayo Special Coagulation Laboratory
Department of Laboratory Medicine and Pathology
Mayo Clinic Rochester;
Instructor, Laboratory Medicine
Mayo Clinic College of Medicine
Rochester, MN, USA
George S. Cembrowski MD, PhD
Director, Medical Biochemistry,
University Hospital
Associate Professor,
Department of Laboratory Medicine and Pathology
University of Alberta
Edmonton, Alberta, Canada
Wayne Chandler MD
Vice Chair
Clinical Coagulation and Chemistry
Department of Pathology and Laboratory Medicine
The Methodist Hospital
Houston, TX, USA
Sindhu Cherian MD
Assistant Professor
Department of Laboratory Medicine
University of Washington
Seattle, WA, USA
Hyun-Sook Chi MD, PhD
Emeritus Professor
University of Ulsan
College of Medicine and Asan Medical Center
Seoul, Korea
James R. Cook MD, PhD
Associate Professor of Pathology
Cleveland Clinic Lerner College of Medicine
Molecular Hematopathology Section Head
Pathology and Laboratory Medicine Institute
Cleveland Clinic
Cleveland, OH, USA
Domnita Crisan MD, PhD
Professor of Pathology
Oakland University William Beaumont School of Medicine
Medical Director, Molecular Pathology
William Beaumont Hospital
Department of Clinical Pathology
Royal Oak, MI, USA
Bruce H. Davis MD
Trillium Diagnostics, LLC,
Bangor, ME, USA
Didier Dhermy MD, PhD
Directeur de Recherche au CNRS
Institut National Transfusion Sanguine
Paris, France
Joseph A. DiGiuseppe MD, PhD
Director, Special Hematology Laboratory
Department of Pathology & Laboratory Medicine
Hartford Hospital
Hartford, CT, USA
Cherie H. Dunphy MD, FCAP, FASCP
Professor of Pathology and Laboratory Medicine
Department of Pathology and Laboratory Medicine
University of North Carolina
Chapel Hill, NC, USA
A. Bradley Eisenbrey MD, PhD
Laboratory Director
Gift of Life Michigan
Ann Arbor, MI, USA
Luis Escribano MD, PhD
Director
Instituto de Estudios de Mastocitosis de Castilla La Mancha
Hospital Virgen del Valle
Toledo, Spain
Emmanuel J. Favaloro PhD FFSc (RCPA)
Principle Hospital Scientist
Department of Haematology
Institute of Clinical Pathology and Medical Research (ICPMR)
Westmead Hospital
Westmead, NSW, Australia
Katherine Galagan MD
Director of Clinical Laboratories and Section Head
Virginia Mason Medical Center
Seattle, WA, USA
Monica V. E. Gallivan MD
Medical Director, Hematology
Quest Diagnostics Nichols Institute
Chantilly, VA, USA
Andrés Garcia-Montero PhD
Researcher
Servicio General de Citometria and Departamento de Medicina
Instituto de Biología Celular y Molecular del Cáncer
Centro de Investigación del Cáncer/IBMCC (CSIC-USAL)
Universidad de Salamanca
Salamanca, Spain
Chris Gardiner PhD, MSc, FIBMS
Postdoctoral Scientist
Nuffield Department of Obstetrics and Gynaecology
University of Oxford
Oxford, UK
Franck Geneviève MD
Laboratoire d’Hématologie
Centre Hospitalier Universitaire
Angers, France
Jérémie Gérard PhD
Doctor of Pharmacy
Laboratoire d’Hématologie
Centre Hospitalier Universitaire
Angers, France
Ian Giles MD
Director of Scientific Affairs
Sysmex America Inc
Mundelein, IL, USA
Piero C. Giordano PhD
Associated Professor
Clinical biochemical molecular geneticist
Center for Human and Clinical Genetics
Leiden Academic Hospital (LUMC)
Leiden, The Netherlands
Eric F. Glassy MD
Affiliated Pathologists Medical Group
Torrance, CA, USA
Alban Godon MD
Laboratoire d’Hématologie
Centre Hospitalier Universitaire
Angers, France
Ralph Green MD, PhD, FRCPath,FCAP, FASCP
Professor and Chair
Department of Medical Pathology and Laboratory Medicine
University of California, Davis
School of Medicine
Sacramento, CA, USA
Laura Green MBBS, MRCP, FRCPath
Consultant in Haemostasis and Transfusion Medicine
Department of Haematology
Barts and The London NHS Trust
London, UK
Bruce Greig MT(ASCP)QCYM
Associate
Department of Pathology, Microbiology, and Immunology
Vanderbilt University
Vanderbilt University Medical Center
Nashville, TN, USA
Andreas Greinacher MD
Full Professor, Department Chair
Institut für Immunologie und Transfusionsmedizin
Ernst-Moritz-Arndt Universität
Greifswald, Germany
Gene Gulati PhD
Professor of Hematology and Associate Director of Hematology Laboratory
Department of Pathology, Anatomy and Cell Biology
Jefferson Medical College of Thomas Jefferson University
Philadelphia, PA, USA
Jin-Yeong Han MD, PhD, FASCP
Professor and Chair
Department of Laboratory Medicine
Dong-A University College of Medicine
Busan, Korea
Paul Harrison BSc, PhD, FRCPath
Clinical Scientist & Honorary Lecturer
Oxford Haemophilia & Thrombosis Centre
Churchill Hospital
Oxford, UK
Henry A. Homburger MD
Consultant (Emeritus), Laboratory Medicine & Pathology
Former Director, Mayo Antibody Immunology Laboratory
Division of Clinical Biochemistry and Immunology
Department of Laboratory Medicine and Pathology
Mayo Clinic Rochester;
Professor (Emeritus), Laboratory Medicine and Pathology
Mayo Clinic College of Medicine
Rochester, MN, USA
Danny Hsu MBBS, FRACP, FRCPA
Senior Registrar in Haematology
Department of Haematology
St George Hospital
Sydney, NSW, Australia
Albert Huisman PhD
Clinical Chemist
University Medical Center Utrecht
Department of Clinical Chemistry and Haematology
Utrecht, The Netherlands
Seongsoo Jang MD, PhD
Assistant Professor
Asan Medical Center
University of Ulsan College of Medicine
Seoul, Korea
Maria Jara-Acevedo MSc
PhD student, Servicio General de Citometría and Departamento de Medicina,
Instituto de Biología Celular y Molecular del Cáncer
Centro de Investigación del Cáncer/IBMCC (CSIC-USAL)
Universidad de Salamanca
Salamanca, Spain
Josep M. Jou MD, PhD
Senior Consultant
Servei Hemoteràpia i Hemostàsia
Hospital Clinic, University of Barcelona
Barcelona, Spain
David Keeling BSc MD FRCP FRCPath
Oxford Haemophilia and Thrombosis Centre
Churchill Hospital
Oxford, UK
Richard Kendall PhD
Director of Global Scientific Affairs
Commercial Director Future Solutions
Abbott Hematology
Santa Clara, CA, USA
Wolfgang Kern MD
Head Immunophenotyping
MLL Munich Leukemia Laboratory
Munich, Germany
Annette S. Kim MD, PhD
Assistant Professor of Pathology, Microbiology and Immunology
Department of Pathology, Microbiology and Immunology
Vanderbilt University Medical Center
Nashville, TN, USA
May-Jean King PhD
Senior Research Biochemist/Clinical Scientist
Membrane Biochemistry
International Blood Group Reference Laboratory
NHS Blood & Transplant
Bristol, UK
Steve Kitchen PhD
Clinical Scientist
Sheffield Haemostasis and Thrombosis Centre
Royal Hallamshire Hospital
Sheffield, UK
Dianne Kitchen PhD
Senior BMS
UK NEQAS for Blood Coagulation
Sheffield, UK
Kandice Kottke-Marchant MD, PhD
Chair, Pathology & Laboratory Medicine Institute
Professor and Chair, Department of Pathology, Cleveland Clinic Lerner College of Medicine
Section Head, Hemostasis and Thrombosis
Dept. of Clinical Pathology
Cleveland Clinic,
Cleveland, OH, USA
Jerry Koutts MD (Syd) BS FRACP FRCPA
Department of Haematology
Institute of Clinical Pathology and Medical Research (ICPMR)
Westmead Hospital
Westmead, NSW, Australia
Francis Lacombe MD, PhD
Hematology Laboratory
Hôpital Haut-Lévêque
Pessac, France
Anton W. Langerak PhD
Associate Professor
Medical Immunologist
Department of Immunology
Erasmus MC
University Medical Center Rotterdam
Rotterdam, The Netherlands
Marlies R. Ledford-Kraemer MBA, BS, MT(ASCP)SH
President, CLOT-ED, Inc.
Emeritus Executive Secretary, NASCOLA
Islamorada, FL, USA
Szu-Hee Lee MBBChir, PhD, FRACP, FRCPA
Professor (Conjoint) and Senior Staff Haematologist
Department of Haematology
St George Hospital
Sydney, NSW, Australia
Qigui Li MD, PhD
Chief of Pharmacokinetics/Pharmacodynamics
Department of Pharmacology
Division of Experimental Therapeutics
Walter Reed Army Institute of Research
Silver Spring, MD, USA
Ying Li MD, PhD
Clinical Associate Professor Department of Pathology and Laboratory Medicine
University of Florida College of Medicine
Gainesville, FL, USA
Samuel J. Machin MD, FRCP, FRCPath
Professor of Haematology, University College London
Consultant Haematologist and Clinical Lead for Laboratory Services
University College London Hospitals
London, UK
Richard A. Marlar PhD
Professor of Pathology
Laboratory Services
Oklahoma City Veterans Administration Medical Center
University of Oklahoma Health Sciences Center
Oklahoma City, OK, USA
Stephen R. Master MD, PhD
Assistant Professor of Pathology and Laboratory Medicine
Department of Pathology and Laboratory Medicine
University of Pennsylvania School of Medicine
Philadelphia, PA, USA
Stefanie L. McFadden MT(ASCP)SH
Clinical Laboratory Consultant
McFadden Laboratory Consulting
Columbus, OH, USA
Sheila McNeill BSMT(ASCP)SH
Clinical Specialist Flow Cytometry
Sentara Norfolk General Hospital
Norfolk, VA, USA
Piet Meijer PhD
Director, ECAT Foundation
Leiden, The Netherlands
Mark A. Micale PhD, FACMG
Associate Professor of Patholgy and Laboratory Medicine
Oakland University William Beaumont School of Medicine
Medical Director
Clinical Cytogenomics Laboratory
Beaumont Health System
Beaumont Laboratory
Royal Oak, MI, USA
R. Scott Miller MD, FIDSA
Division of Experimental Therapeutics
Walter Reed Army Institute of Research
Silver Spring, MD, USA
Laila Mnayer PhD, FACMG
Director of Molecular Pathology and Cytogenetics
Department of Pathology and Laboratory Medicine
Hartford Hospital
Hartford, CT, USA
Anwar N. Mohamed MD, FACMG
Professor of Pathology
Department of Pathology/Cytogenetic Laboratory
Wayne State University School of Medicine/Detroit Medical Center
Detroit, MI, USA
José Mario Morgado MSc
Master in Science, Biology Researcher
Instituto de Estudios de Mastocitosis de Castilla La Mancha
Hospital Virgen del Valle
Toledo, Spain
Gurunathan Murugesan PhD
Pathology and Laboratory Medicine Institute
Cleveland Clinic
Cleveland, OH, USA
William L. Nichols MD
Consultant, Hematology & Internal Medicine, and Laboratory Medicine & Pathology
Mayo Special Coagulation Laboratory and Coagulation Clinical Centers
Divisions of Hematopathology and Hematology
Mayo Clinic Rochester;
Associate Professor, Medicine and Laboratory Medicine
Mayo Clinic College of Medicine
Rochester, MN, USA
Teri Oldaker MLS (ASCP), QCYM
Senior Director of Flow Cytometry
Genoptix Medical Laboratory
Carlsbad, CA, USA
John Olson MD, PhD
Professor and Vice Chair for Clinical Affairs
Department of Pathology
The University of Texas Health Science Center
Director of Clinical Laboratories
University Health System
San Antonio, TX, USA
Alberto Orfao MD, PhD
Professor, Servicio General de Citometría, and Departamento de Medicina
Instituto de Biología Celular y Molecular del Cáncer
Centro de Investigación del Cáncer/IBMCC (CSIC-USAL)
Universidad de Salamanca
Salamanca, Spain
Powers Peterson MD
Formerly: Associate Professor of Pathology & Laboratory Medicine
Weill Cornell Medical College in Qatar
New York, NY, USA;
Consultant in Laboratory Medicine and Pathology
Hamad Medical Corporation
Doha, Qatar
Currently: Medical Director
Quest Diagnostics Nichols Institute
Valencia, CA, USA
Norman B. Purvis, Jr. PhD
Senior Director, Development
Nodality, Inc.
South San Francisco, CA, USA
Alexandra Rice FRCPath
Consultant Histopathologist
Royal Brompton Hospital
London, UK
Stephen J. Richards PhD FRCPath
Consultant Clinical Scientist
Haematological Malignancy Diagnostic Service
Department of Haematology
St James’s Institute of Oncology
Leeds Teaching Hospitals NHS Trust
Leeds, UK
Bill G. Richendollar MD
Staff Pathologist
De Kalb Medical
Decatur, GA, USA
Kathleen Richkind PhD, FACMG
Clinical Cytogeneticist
Genzyme Genetics
Santa Fe, NM, USA
Laura Sanchez-Muñoz MD, PhD
Instituto de Estudios de Mastocitosis de Castilla La Mancha
Hospital Virgen del Valle
Toledo, Spain
Linda M. Sandhaus MD, MS
Associate Professor of Pathology
University Hospitals Case Medical Center
Cleveland, OH, USA
Susanne Schnittger PhD
Head of Molecular Genetics
MLL Munich Leukemia Laboratory
Munich, Germany
Sixten Selleng MD
Consultant in Anaesthesia
Klinik für Anasthesiologie und Intensivmedizin
Ernst-Moritz-Arndt Universität
Greifswald, Germany
Katerine Seywerd MD
Pathology Resident
Department of Pathology
University of Oklahoma Health Sciences Center
Oklahoma City, OK, USA
Elkin Simson MD, FCAP, FASCP
Consultant in Hematology, Laboratory Automation and Laboratory Informatics
Clinical Associate Professor, Mount Sinai School of Medicine
New York, NY, USA
Michael Spannagl MD, PhD
Hemostasis and Transfusion Medicine
University Hospital
Munich, Germany
Maryalice Stetler-Stevenson MD, PhD
Chief, Flow Cytometry Unit
Laboratory of Pathology, CCR, NCI, NIH
Bethesda, MD, USA
Tomasz Szczepaski MD, PhD
Professor, Head of the Department
Department of Pediatric Hematology and Oncology
Medical University of Silesia
Zabrze, Poland
Cristina Teodosio MSc
PhD student, Servicio General de Citometria and Departamento de Medicina
Instituto de Biología Celular y Molecular del Cáncer
Centro de Investigación del Cáncer/IBMCC (CSIC-USAL)
Universidad de Salamanca
Salamanca, Spain
Karl S. Theil MD
Staff Pathologist
Department of Clinical Pathology
Cleveland Clinic
Cleveland, OH, USA
Raymond R. Tubbs DO
Head, Section of Molecular Oncologic Pathology
Department of Molecular Pathology
Pathology and Laboratory Medicine Institute
Cleveland Clinic;
Professor of Pathology
Cleveland Clinic Lerner College of Medicine
Cleveland, OH, USA
Jacques J. M. van Dongen MD, PhD
Professor of Immunology
Department of Immunology
Erasmus MC
University Medical Center Rotterdam
Rotterdam, The Netherlands
H. W. Verbruggen PhD
University Medical Centre St. Radboud
Central Laboratory for Hematology
Nijmegen
The Netherlands
Ilka Warshawsky MD, PhD
Staff Pathologist
Department of Molecular Pathology
Cleveland Clinic Foundation
Cleveland, OH, USA
Peter J. Weina PhD, MD, FACP, FIDSA
Headquarters
Walter Reed Army Institute of Research
Silver Spring, MD, USA
Brent L. Wood MD, PhD
Professor, Hematopathology Laboratory
Department of Laboratory Medicine
University of Washington
Seattle, WA, USA
Sophia Yohe MD
Assistant Professor
Department of Laboratory Medicine and Pathology
Divisions of Hematopathology and Molecular Diagnostic Pathology
University of Minnesota Medical Center
Minneapolis, MN, USA
Constance Yuan MD, PhD
Staff Clinician
Laboratory of Pathology, NCI, NIH
Bethesda, MD, USA
Marc Zandecki PhD
Professor of Hematology
Laboratoire d’Hématologie
Centre Hospitalier Universitaire
Angers, France
Preface
Hematologic disorders range from some of the most common medical conditions, like anemia and leukocytosis, to some of the most rare and perplexing conditions, like inherited thrombocytopenia and mast cell disorders. Yet, due to the relatively easy accessibility of blood samples, hematology is a rapidly evolving area of medical knowledge that has seen a virtual explosion of diagnostic laboratory testing and therapeutics in the past decade. Common diagnostic methods range from visual peripheral blood and bone marrow morphology to flow cytometric analysis of erythrocytes, leukocytes and platelets, to functional testing of hemostasis proteins and platelets, to electrophoretic hemoglobin analysis and molecular DNA and RNA analysis of leukemias and lymphomas.
Laboratory Hematology Practice is a new textbook focused on a practical approach to understanding the diagnostic utility of new hematology laboratory testing, how to critically assess new testing technology and how to implement, interpret and manage new assays and new technology in the laboratory. Most hematology textbooks focus on disease pathophysiology and patient management, but gloss over the details of laboratory diagnosis, an important aspect of patient care. Laboratory testing is complex and there are many details of accurate laboratory testing, from method selection to assay validation, quality control, results reporting and interpretation. Thus we chose to focus on diagnostic methodologies for this book and structured it along technology lines instead of by disease category. Technologies covered include cellular analysis, flow cytometry, molecular diagnostics, bone marrow analysis, hemostasis and thrombosis testing, special hematology techniques, point-of-care testing and information management. Emphasis is placed upon the use of established and emerging technologies in the diagnosis of hematologic disorders, but also includes operational aspects of laboratory testing, such as proper sample collection, quality control, informatics and laboratory management. Unique features of Laboratory Hematology Practice are incorporation of international standards for laboratory practice, methods for evaluation of testing technology, and on-line access to book content and figures. We hope that Laboratory Hematology Practice will provide practical information and fill a niche in the field as it will help laboratorians critically understand, assess, implement and manage a wide variety of hematologic technologies ranging from cellular analysis to hemostasis testing and molecular diagnostics. We hope this text will become a welcome addition to the library of every practicing hematologist, hematopathologist, laboratory scientist in hematology, and trainees in these areas of medical and laboratory practice.
This project was sponsored by the International Society for Laboratory Hematology (ISLH). The ISLH arose out of a series of symposia on “Technological Innovations in Laboratory Hematology” organized by Dr Berend Houwen that started in Lake Louise, Canada in 1983. Dr Houwen and Dr Bruce Davis then led an international group of laboratory professionals in the formation of ISLH in 1992. In addition to Drs Houwen and Davis, the original founding ISLH Directors were Dr Brian Bull, Dr Ralph Green, Professor Samuel Machin, Dr Robert Pierre, Dr Elkin Simson and Dr Noriyuki Tatsumi. The goal of ISLH is “to further the interests of laboratory hematology as a professional activity and to provide a forum for its members for the exchange of new ideas and information on the subject of laboratory hematology”. It is the only international society specifically focused on the technological and laboratory aspects of hematology. Since 1992, the ISLH has grown to an international organization that holds annual meetings around the globe, with over 700 members from over 50 countries. The ISLH has developed strong affiliations with many national hematology societies. The ISLH originated as a society focused on aspects of cellular analysis and has grown to incorporate dissemination of knowledge in hemostasis and thrombosis, hematologic molecular diagnosis, bone marrow morphology, standards and guidelines, hematology informatics, hemoglobinopathies and point-of-care testing. The ISLH publishes a scientific journal, the International Journal of Laboratory Hematology. Given the focus on technological advances in laboratory hematology, it is truly fitting that this textbook, Laboratory Hematology Practice, should be sponsored by the ISLH.
It is with gratitude and respect that we pay tribute and dedicate this book to two of the original ISLH Directors who have passed away, Dr Berend Houwen and Dr Noriyuki Tatsumi. Dr Berend Houwen (1941–2004), the co-founder of ISLH, was most recently Corporate Medical Director of Beckman Coulter, preceded by Associate Professor of Pathology at Loma Linda University and Associate Professor of Pathology & Medicine at the University of Calgary. Berend was a man of vision and determination, who was a champion for laboratory hematology and published widely on many aspects of laboratory hematology testing, most notably flow cytometry, microcytic anemia, hematology testing errors, red cell volume distribution and red cell indices. Dr Noriyuki Tatsumi (1937–2010) was most recently Professor of Clinical and Laboratory Medicine at Osaka City University. Dr Tatsumi was a leader in standardization and quality assurance of hematological testing and carried out a wide range of investigation in the field of laboratory hematology. In addition to many years of dedication to ISLH, he founded the Japanese Society for Laboratory Hematology (JSLH) in 2000. These two original ISLH Directors exemplify the passion for laboratory hematology and for lifelong research and education, which we hope pervades every page of this textbook.
Kandice Kottke-Marchant, MD, PhD
Bruce H. Davis, MD
7/1/11